D/E Neutralising Broth is used to neutralise and determine the bactericidal activity of antiseptics and disinfectants. D/E Neutralising Broth without Tween is not intended for use in the diagnosis of disease or other conditions in humans.

D/E Neutralising Broth was developed by Dey and Engley to neutralise a broad spectrum of disinfectants and preservative antimicrobial chemicals, including quaternary ammonium compounds, phenolics, iodine, chlorine preparations, mercurials, formaldehyde, and glutaraldehyde. D/E Neutralising media neutralise higher concentrations of residual antimicrobials when compared with other standard neutralising formulations, such as Letheen media, Thioglycollate media, and Neutralising Buffer. Total neutralisation of disinfectants is critical. Disinfectant residues can result in a false-negative (no-growth) test. D/E Neutralising Broth without Tween effectively neutralises the inhibitor action of disinfectant carryover, allowing differentiation between bacteriostasis and the true bactericidal action of disinfectant chemicals. This is a critical characteristic to consider when evaluating a disinfectant. D/E Neutralising Broth without Tween is recommended for use in disinfectant evaluations, environmental sampling (swab and contact plate methods), and testing of water-miscible cosmetics.

• Analyte: Non-selective
• Platform: dehydrated culture media
• Formula: enzymatic digest of casein 5.0g/L, yeast extract 2.5g/L, dextrose (glucose) 10.0g/L, sodium thioglycollate 1.0g/L, sodium thiosulfate 6.0g/L, sodium bisulfite 2.5g/L, lecithin 7.0g/L, bromocresol purple 0.02g/L
• Grams per litre: 34.00
• Litres per 500g: 13.00